NCI-H460-Luc
CBP30033L
| I. Introduction | |||||||||||
| Host Cell: | NCI-H460 | ||||||||||
| Expressed gene: | Luciferase | ||||||||||
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||||||||||
| Freeze Medium: | 90% FBS+10% DMSO | ||||||||||
| Culture Medium: | RPMI-1640+10%FBS+2μg/ml Puromycin | ||||||||||
| Mycoplasma Status: | Negative | ||||||||||
| Storage: | Liquid nitrogen | ||||||||||
| II. Description of Host Cell Line | |||||||||||
| Organism: | Homo sapiens,human | ||||||||||
| Tissue: | Lung:pleural effusion | ||||||||||
| Disease: | Carcinoma;large cell lung cancer | ||||||||||
| Morphology: | Epitheloid cell | ||||||||||
| Growth Properties: | Adherent | ||||||||||
| III. Representative Data | |||||||||||
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Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly recommend purchasing from Cobioer). The number of Luminescence signal is 475±15(Mean±SD) on NCI-H460 cells while 902713±23711(Mean±SD) on NCI-H460-Luc cells. |
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| IV. Stablity Test | |||||||||||
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Figure 2. Stability test results. NCI-H460-Luc cells showed at least 32 passage stability with similar luminescence unit. |
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| V. In VIVO Data | |||||||||||
Intracranial injection：
1. Weight Change 2. Total Flux
 Figure 4. Total flux NCI-H460-Luc. 肺原位： |
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Index |
Content |
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Animal |
BALB/c-nude |
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Implanted Position |
肺原位 |
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Injected Cell Numbers |
5×10^6/50μL |
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Matrix gel |
Without matrix gel |
1.Tumor weight
Figure 5. NCI-H460-Luc Orthotopic Tumor Model.
2. Tumor flux
Figure 6. NCI-H460-Luc Orthotopic Tumor Model.
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
